Why fbs has to be heat inactivated

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Sci Adv. When one heat inactivates serum, not only is complement protein degraded, which has been indicated above may not be necessary , but also all other components such as amino acids, vitamins, growth factors, etc. In many cases, certain important components that are of borderline concentration may be reduced to concentrations less than that required by certain cell lines for growth.

If it is experimentally determined that heat inactivation is required, however, the most common procedure and the one recommended by the TCF is as follows:. In summary, there may be few reasons to heat inactivate serum. Those reasons should be experimentally determined and documented. In most cases heat inactivation does not improve growth promotion and may actually have adverse effects.

Also, heat inactivation can cause an increase in the formation of precipitates that are often mistaken as microbial contamination.

If heat inactivation is necessary, strictly follow a repeatable procedure. Consistency in the way serum is thawed, handled, and stored can play a significant role in consistency of cell growth and repeatability in experimentation. If your laboratory normally stocks heat-inactivated serum, you can probably use it without a problem. In fact, many of the labs that use Coriell cell lines do use heat-inactivated serum. All rights reserved.

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